Epitope mapping of SARS-CoV-2 spike protein differentiates the antibody binding activity in vaccinated and infected individuals
نویسندگان
چکیده
Previous studies have attempted to characterize the antibody response of individuals SARS-CoV-2 virus on a linear peptide level by utilizing microarrays. These helped identify epitopes that potential be used for diagnostic tests infected individuals. The immunological responses who received two most popular vaccines available in US, Moderna mRNA-1273 or Pfizer BNT162b2 mRNA vaccines, not been characterized. We aimed peptides spike protein elicited high IgG IgA binding activity and compare immunoreactivity those both doses either vaccine Our results revealed significant among recently Some these are located near variable regions receptor domains as well conserved region c-terminal implicated infectivity SARS-CoV-2. Vaccinated lacked distinct markers despite overall being similar.
منابع مشابه
Evolutionary Analysis of Mammalian ACE2 and the Key Residues Involved in Binding to the Spike Protein Revealed Potential SARS-CoV-2 Hosts
Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spilled over to humans via wild mammals, entering the host cell using angiotensin-converting enzyme 2 (ACE2) as receptor through Spike (S) protein binding. While SARS-CoV-2 became fully adapted to humans and globally spread, some mammal species were infected back. The present study evaluated the potential risk of mammals...
متن کاملGastrointestinal Manifestations in Patients Infected with SARS-CoV-2
Coronavirus disease 2019, caused by severe acute respiratory syndrome coronavirus 2, has recently become the number one problem affecting global health. Coronavirus disease 2019 is principally recognized by its respiratory manifestations; however, recent studies have shown an increasing number of patients with gastrointestinal complaints like diarrhea, nausea, vomiting, and abdominal discomfort...
متن کاملSARS-CoV Infection in Vaccinated Ferrets
Miriam E. R. Darnell, Ewan P. Plant, Hisayoshi Watanabe, Russ Byrum, Marisa St. Claire, Jerrold M. Ward, and Deborah R. Taylor Laboratory of Hepatitis and Related Emerging Agents, Division of Emerging and Transfusion-Transmitted Diseases, Office of Blood Research and Review, and Laboratory of Hepatitis Viruses, Center for Biologics Evaluation and Research, US Food and Drug Administration, and C...
متن کاملThe Epitope Study on the SARS-CoV Nucleocapsid Protein
The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA), the recombinant N proteins and the synthesized peptides derived from the N protein were sc...
متن کاملAntimyogenic Effect of SARS-CoV Spike Protein in C2C12 Myoblasts
C2C12 myoblasts serve as well-established model system to study myogenesis, as they fuse to form multinucleated myotubes. Severe acute respiratory syndrome coronavirus (SARS–CoV) spike (S) protein plays a crucial role in viral entry. Exogenous expression of S protein in C2C12 myoblasts inhibits the formation of myotubes. Global changes in gene expression were studied in C2C12 cells expressing S...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in virology
سال: 2023
ISSN: ['2673-818X']
DOI: https://doi.org/10.3389/fviro.2023.988109